Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
This antibody does not appear to be useful for indirect immunofluorescence under these experimental conditions in HeLa as only background staining was observed at any stage of the cell cycle. Both Methanol and paraformaldehyde fixed cells were examined at various dilutions (1:100 & 1:200) for either 1hr or overnight (16hrs).
Abcam response
Thank you for your valuable feedback. ICC/IF was not a tested application for ab46783 and it seems that this antibody does not recognize Aurora C under these experimental conditions. Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
提交于 Jul 02 2007