Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (anal cancer)
Specification
anal cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Blocking step
Sequential pre-diluted peroxidase (10 min) and protein (10 min) blocks. as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Dilution
1/200
Incubation time
40 minute(s) · Temperature: 20°C · Diluent: antibody diluent
Secondary antibody
Name
Non-Abcam antibody was used: Gaot anti-mouse HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Stage and grade of cancer is unknown
Blocking was with ready-to-use peroxidase block followed by protein block - both pre-diluted and manufactured by Dako
Blocking was with ready-to-use peroxidase block followed by protein block - both pre-diluted and manufactured by Dako
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
提交于 Jul 17 2008