Anti-IMPDH1抗体(ab33039)
Key features and details
- Rabbit polyclonal to IMPDH1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IMPDH1抗体 -
描述
兔多克隆抗体to IMPDH1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Xenopus laevis, Zebrafish -
免疫原
Synthetic peptide corresponding to Human IMPDH1 aa 500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab33038) -
常规说明
The immunogen used for this product shares 86% homology with IMPDH2. Cross-reactivity with this protein has not been confirmed experimentally.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab33039于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
说明 |
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WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. -
组织特异性
IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor. -
通路
Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1. -
疾病相关
Defects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10) [MIM:180105]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant. -
序列相似性
Belongs to the IMPDH/GMPR family.
Contains 2 CBS domains. - Information by UniProt
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数据库链接
- Entrez Gene: 3614 Human
- Entrez Gene: 23917 Mouse
- Entrez Gene: 362329 Rat
- Omim: 146690 Human
- SwissProt: P20839 Human
- SwissProt: P50096 Mouse
- SwissProt: D3ZLZ7 Rat
- Unigene: 654401 Human
see all -
别名
- IMDH1_HUMAN antibody
- IMP (inosine monophosphate) dehydrogenase 1 antibody
- IMP dehydrogenase 1 antibody
see all
图片
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All lanes : Anti-IMPDH1 antibody (ab33039) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat anti-rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa -
All lanes : Anti-IMPDH1 antibody (ab33039) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab33039 被引用在 5 文献中.
- Zhao JZ et al. MYBL2 regulates de novo purine synthesis by transcriptionally activating IMPDH1 in hepatocellular carcinoma cells. BMC Cancer 22:1290 (2022). PubMed: 36494680
- Xu X et al. Rewiring of purine metabolism in response to acidosis stress in glioma stem cells. Cell Death Dis 12:277 (2021). PubMed: 33723244
- Feng X et al. UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism. EMBO J 39:e102541 (2020). PubMed: 31975428
- Dang W et al. Inhibition of Calcineurin or IMP Dehydrogenase Exerts Moderate to Potent Antiviral Activity against Norovirus Replication. Antimicrob Agents Chemother 61:N/A (2017). PubMed: 28807916
- Kim HR et al. A p53-inducible microRNA-34a downregulates Ras signaling by targeting IMPDH. Biochem Biophys Res Commun 418:682-8 (2012). WB ; Human . PubMed: 22301190