Application
ELISA
Sample
Human Recombinant protein (Human IL-8 from R&D Systems)
Specification
Human IL-8 from R&D Systems
Type
Sandwich (Capture)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 1xPBS 0.05% Tween-20 1% BSA
Dilution
1/500
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Signal was detected by using a Biotin labelled detection antibody (Mouse Monoclonal anti-IL-8). Incubation at room temperature for 1 h. Incubation with Streptavidin-HRP for 20 min at room temperature, followed by incubation with TMB for 15 min at room temperature. Reaction was stopped with 1M H2SO4.
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提交于 Mar 07 2014