Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Macrophages cell line)
Permeabilization
Yes - 0.1% triton-X100, in 3%BSA in PBS
Specification
Macrophages cell line
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA in PBS
Dilution
1/100
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: sc-2012 Goat polyclonal
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Varkha Agrawal
Verified customer
提交于 Sep 11 2012