- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-IL1 alpha抗体
参阅全部 IL1 alpha 一抗 -
描述山羊多克隆抗体to IL1 alpha
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宿主Goat
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经测试应用适用于: WB, Sandwich ELISA, Neutralising, IHC-FoFrmore details
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种属反应性与反应: Rat
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免疫原
Highly pure (>98%) recombinant rIL-1a (rat Interleukin-1-alpha).
性能
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形式Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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存储溶液PBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型unknown
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轻链类型unknown
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab9875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.2 µg/ml. | |
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to IL1 alpha (Biotin) (ab84012). Using ab84012 as the detection antibody and ab109792 as the capture antibody, the detection limit for recombinant Rat IL1 alpha is 0.2 - 0.4 ng/well | |
| Neutralising | Use a concentration of 0.011 - 0.017 µg/ml. Yields one-half maximal inhibition [ND50] of the biological activity of Rat IL1 alpha (50 pg/ml). | |
| IHC-FoFr | Use at an assay dependent dilution. |
靶标
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功能Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
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序列相似性Belongs to the IL-1 family.
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结构域The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
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细胞定位Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
- Information by UniProt
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数据库链接
- Entrez Gene: 24493 Rat
- SwissProt: P16598 Rat
- Unigene: 12300 Rat
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别名
- BAF antibody
- FAF antibody
- Hematopoietin 1 antibody
see all
图片
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-IL1 alpha antibody (ab9875)Image from Johnson EA & Kan RK, J Neuroinflammation. 2010 Jul 22;7:40. Fig 5.; doi:10.1186/1742-2094-7-40; 22 July 2010, Journal of Neuroinflammation 2010, 7:40Immunohistochemical analysis of rat brain tissue, staining IL1 alpha in microglia, with ab9875 (1/1000). An AlexaFluor®-conjugated anti-goat IgG was used as the secondary antibody. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-IL1 alpha antibody (ab9875)This image is courtesy of the Tissue Profiling group, SciLifeLab StockholmImmunohistochemical analysis of PFA perfusion fixed frozen rat brain, staining IL1 alpha with ab9875 at 1 µg/ml. A peroxidase conjugated secondary antibody was added and staining was detected using a fluorescein Tyramide Signal Amplification (TSA™) reagent. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-IL1 alpha antibody (ab9875)This image is courtesy of the Tissue Profiling group, SciLifeLab StockholmImmunohistochemical analysis of PFA perfusion fixed frozen rat brain, staining IL1 alpha with ab9875 at 1 µg/ml. A peroxidase conjugated secondary antibody was added and staining was detected using a fluorescein Tyramide Signal Amplification (TSA™) reagent.
文献
This product has been referenced in:
- Johnson EA & Kan RK The acute phase response and soman-induced status epilepticus: temporal, regional and cellular changes in rat brain cytokine concentrations. J Neuroinflammation 7:40 (2010). IHC-FoFr ; Rat . Read more (PubMed: 20649973) »
客户评价及客户问答
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