重组HRP Anti-IKK beta抗体[Y466] (ab194713)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [Y466] to IKK beta
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
概述
-
产品名称
HRP Anti-IKK beta抗体[Y466]
参阅全部 IKK beta 一抗 -
描述
HRP兔单克隆抗体[Y466] to IKK beta -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Daudi cell lysate and human cervical carcinoma. IHC-P: FFPE human breast adenocarcinoma.
-
常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y466 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab194713于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
|
说明 |
---|
WB
1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa). |
靶标
-
功能
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3. -
组织特异性
Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ. -
细胞定位
Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
-
数据库链接
- Entrez Gene: 3551 Human
- Entrez Gene: 16150 Mouse
- Omim: 603258 Human
- SwissProt: O14920 Human
- SwissProt: O88351 Mouse
- Unigene: 597664 Human
- Unigene: 277886 Mouse
-
别名
- I kappa B kinase 2 antibody
- I kappa B kinase beta antibody
- I-kappa-B kinase 2 antibody
see all
图片
-
All lanes : HRP Anti-IKK beta antibody [Y466] (ab194713) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : IKBKB (IKK beta) knockout HAP1 whole cell lysate
Lysates/proteins at 1/20 dilution per lane.
Predicted band size: 87 kDa
Observed band size: 87 kDa
Exposure time: 20 minutesab194713 was shown to recognize IKK beta in wild-type HAP1 cells as signal was lost at the expected MW in IKBKB (IKK beta) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and IKBKB (IKK beta) knockout samples were subjected to SDS-PAGE. Ab194713 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
-
HRP Anti-IKK beta antibody [Y466] (ab194713) at 1/5000 dilution + Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 87 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% milk before being incubated with ab194713 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (0)
ab194713 尚未被引用在任何文献中。