Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk (TBS-tween)
Dilution
1/1000
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
8 minute(s) and 42 second(s)
Bands
Specific: 19 kDa Non-specific: 31-50 kDa
Additional data
Abcam response
Any feedback provided by our customers is much appreciated. For users wishing to test our products in untested species or applications, we would encourage them to participate in our Abtrial program, by which you will beneficiate from a discount off your next purchase for the value of the product tested by submitting your results to our website. The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 16 2016