Application
ChIP
Sample
Human Cell lysate - nuclear (HT1080)
Negative control
Multiple sites that are in gene poor regions and are not thought to bind based on studies in other species.
Specification
HT1080
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control
H3K9 trimethylation antibody. The Actin promoter and multiple genes that are known to be bound by NIPBL in other organisms.
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
Additional Notes
Was unable to obtain any enrichment with this antibody in crosslinked chip. Pulled down approx 0.004% of input, however no difference in regions thought to be bound by NIPBL and those unbound. Similar amount pulled down by Rat IgG.
Abcam response
Thank you for submitting this abreview. Abcam have not previously tested ab106768 in ChIP and from this abreview, it seems that the antibody does not work in this application, under these conditions. We would welcome further feedback from other customers using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Leisha Nolen
Verified customer
提交于 Dec 05 2011