Anti-NIPBL抗体[KT55] (ab106768)
Key features and details
- Rat monoclonal [KT55] to NIPBL
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
概述
-
产品名称
Anti-NIPBL抗体[KT55]
参阅全部 NIPBL 一抗 -
描述
大鼠单克隆抗体[KT55] to NIPBL -
宿主
Rat -
特异性
Recognises Human and Mouse NIPBL isoform B (short form).
-
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human NIPBL (C terminal).
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
-
纯度
Protein G purified -
纯化说明
Purified from culture supernatant using a protein G column. -
克隆
单克隆 -
克隆编号
KT55 -
同种型
IgG2a -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106768于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use 1µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
---|
Flow Cyt
Use 1µg for 106 cells. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
Probably plays a structural role in chromatin. Involved in sister chromatid cohesion, possibly by interacting with the cohesin complex. -
组织特异性
Widely expressed. Highly expressed in heart, skeletal muscle, fetal and adult liver, fetal and adult kidney. Expressed at intermediates level in thymus, placenta, peripheral leukocyte and small intestine. Weakly or not expressed in brain, colon, spleen and lung. -
疾病相关
Cornelia de Lange syndrome 1 -
序列相似性
Belongs to the SCC2/Nipped-B family.
Contains 5 HEAT repeats. -
发展阶段
In embryos, it is expressed in developing limbs and later in cartilage primordia of the ulna and of various hand bones. Sites of craniofacial expression include the cartilage primordium of the basioccipital and basisphenoid skull bones and elsewhere in the head and face, including a region encompassing the mesenchyme adjacent to the cochlear canal. Also expressed in the spinal column, notochord and surface ectoderm sclerotome and what seem to be migrating myoblasts. Expressed in the developing heart in the atrial and ventricular myocardium and in the ventricular tubeculae but absent in the endocardial cushions. Also expressed in the developing esophagus, trachea and midgut loops, in the bronchi of the lung and in the tubules of the metanephros. Expression in organs and tissues not typically affected in CDL (e.g. the developing trachea, bronchi, esophagus, heart and kidney) may reflect a bias towards underreporting of more subtle aspects of the phenotype or problems that typically present later in life. Expressed in the mesenchyme surrounding the cochlear canal possibly reflecting the hearing impairment commonly found. Weakly or not expressed in embryonic brain. -
结构域
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 25836 Human
- Omim: 608667 Human
- SwissProt: Q6KC79 Human
- Unigene: 481927 Human
-
形式
There are 3 isoforms produced by alternative splicing. Isoform 1 also known as: A; IDN3-A. Isoform 2 also known as: B; IDN3-B. -
别名
- CDLS antibody
- Colon tumor susceptibility 2 antibody
- Delangin antibody
see all
图片
-
Overlay histogram showing HepG2 cells stained with ab106768 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab106768, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rat IgG (H+L) (ab150165) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab106768 被引用在 3 文献中.
- Leylek TR et al. Reduced Expression of Genes Regulating Cohesion Induces Chromosome Instability that May Promote Cancer and Impact Patient Outcomes. Sci Rep 10:592 (2020). PubMed: 31953484
- Meisenberg C et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). PubMed: 30554942
- Nolen LD et al. Regional chromatin decompaction in Cornelia de Lange syndrome associated with NIPBL disruption can be uncoupled from cohesin and CTCF. Hum Mol Genet 22:4180-93 (2013). WB ; Human . PubMed: 23760082