Application
Flow Cytometry
Sample
Rhesus monkey Cell (choroidal endothelial cells)
Permeabilization
No
Gating Strategy
size and granularity
Specification
choroidal endothelial cells
Preparation
Cell harvesting/tissue preparation method: cells were detached from tissue culture plates with Accutase
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Fixation
Paraformaldehyde
Other product details
Incubation time
20 minute(s) · Temperature: 4°C · Diluent: 2mM EDTA + 0.5% BSA in PBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Vector Laboratories DyLight 488 Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Abcam response
Thank you for testing ab7815 in an untested application (flow cytometry) by participating to our AbTrial program (www.abcam.com/abtrial).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 14 2017