重组Anti-ICAD抗体[EPR4220] (ab108924)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4220] to ICAD
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ICAD抗体[EPR4220]
参阅全部 ICAD 一抗 -
描述
兔单克隆抗体[EPR4220] to ICAD -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat cell lysate, HeLa cell lysate; human breast carcinoma tissue; HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR4220 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108924于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/250 - 1/500.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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ICC/IF
1/250 - 1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Inhibitor of the caspase-activated DNase (DFF40). -
序列相似性
Contains 1 CIDE-N domain. -
翻译后修饰
Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1676 Human
- Omim: 601882 Human
- SwissProt: O00273 Human
- Unigene: 484782 Human
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别名
- A330085O09Rik antibody
- Caspase activated deoxyribonuclease inhibitor short form antibody
- DFF 1 antibody
see all
图片
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All lanes : Anti-ICAD antibody [EPR4220] (ab108924) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg/ml per lane.
Predicted band size: 37 kDa -
Immunohistochemical staining of paraffin-embedded human breast carcinoma using ab108924 at a diltion of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescent staining of HeLa cells using ab108924 at a dilution of 1/250.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108924 被引用在 1 文献中.
- Zhu CH et al. Investigation on the mechanisms of human sperm DNA damage based on the proteomics analysis by SWATH-MS. Clin Proteomics 20:2 (2023). PubMed: 36609216