人Von Willebrand Factor ELISA试剂盒(VWF) (ab108918)
Key features and details
- Sensitivity: 0.38 mIU/ml
- Range: 0.625 mIU/ml - 40 mIU/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Von Willebrand Factor ELISA试剂盒(VWF)
参阅全部 Von Willebrand Factor 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.6% 批次间 样品 n Mean SD CV% Overall 9.6% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.38 mIU/ml -
范围
0.625 mIU/ml - 40 mIU/ml -
回收率
98 %
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检测时间
5h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human Von Willebrand Factor (VWF) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Von Willebrand Factor in plasma, serum, and cell culture supernatants.
A Von Willebrand Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Von Willebrand Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Von Willebrand Factor captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 100X Biotinylated Human Von Williebrand Factor Antibody 1 x 60µl Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml Von Williebrand Factor Microplate (12 x 8 well strips) 1 unit Von Williebrand Factor Standard 1 vial -
研究领域
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功能
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. -
组织特异性
Plasma. -
疾病相关
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
结构域
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. -
翻译后修饰
All cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated. -
细胞定位
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules. - Information by UniProt
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别名
- Coagulation factor VIII
- Coagulation factor VIII VWF
- F8VWF
see all -
数据库链接
- Entrez Gene: 7450 Human
- Omim: 613160 Human
- SwissProt: P04275 Human
- Unigene: 440848 Human
图片
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab108918 被引用在 23 文献中.
- Li H et al. Serum levels of laminin and von Willebrand factor in COVID-19 survivors 6 months after discharge. Int J Infect Dis 115:134-141 (2022). PubMed: 34843955
- Oldfield L et al. Blood levels of adiponectin and IL-1Ra distinguish type 3c from type 2 diabetes: Implications for earlier pancreatic cancer detection in new-onset diabetes. EBioMedicine 75:103802 (2022). PubMed: 34990893
- Bian J et al. Relationship between Serum FGF21 and vWF Expression and Carotid Atherosclerosis in Elderly Patients with Hypertension. J Healthc Eng 2022:6777771 (2022). PubMed: 35242298
- Hultström M et al. Angiopoietin-2 Inhibition of Thrombomodulin-Mediated Anticoagulation-A Novel Mechanism That May Contribute to Hypercoagulation in Critically Ill COVID-19 Patients. Biomedicines 10:N/A (2022). PubMed: 35740360
- Cui W et al. Acrolein Induces Systemic Coagulopathy via Autophagy-dependent Secretion of von Willebrand Factor in Mice after Traumatic Brain Injury. Neurosci Bull 37:1160-1175 (2021). PubMed: 33939120