人VEGFC ELISA试剂盒(ab100664)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 13.72 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人VEGFC ELISA试剂盒
参阅全部 VEGFC 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 15 pg/ml -
范围
13.72 pg/ml - 10000 pg/ml -
回收率
98 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 90.46 83% - 99% Serum 109.4 100% - 118% Plasma 96.02 86% - 104% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s VEGFC Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGFC in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human VEGFC coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGFC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGFC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGFC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human VEGFC 2 vials Recombinant Human VEGFC Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml VEGFC Microplate (12 x 8 wells) 1 unit -
研究领域
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相关性
Vascular endothelial growth factors (VEGFs), also known as vasculotropins, are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. The target cell specificity of VEGF is restricted to vascular endothelial cells. Vascular Endothelial Growth Factor C (VEGFC) is a member of the VEGF subfamily of PDGF-related growth factors. It is the ligand for Flt4 (VEGFR3) and KDR (VEGFR2). VEGFC binds Flt4 and induces tyrosine autophosphorylation of VEGFR3 and VEGFR2. VEGFC also stimulates the migration of bovine capillary endothelial cells in collagen gel. It is a specific growth factor for the lymphatic vascular system and mediates lymphangiogenesis. VEGFC is abundantly expressed in heart and skeletal muscle. Other tissues such as lung and kidney also express VEGFC. -
别名
- Flt4 L
- Flt4 ligand
- FLT4 ligand DHM
see all -
数据库链接
- SwissProt: P49767 Human
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab100664 被引用在 1 文献中.
- He X et al. MicroRNA-186 regulates the invasion and metastasis of bladder cancer via vascular endothelial growth factor C. Exp Ther Med 14:3253-3258 (2017). PubMed: 28966690