人TNFRSF18 ELISA试剂盒(GITR) (ab100528)

概述

  • 产品名称
    人TNFRSF18 ELISA试剂盒(GITR)
    参阅全部 TNFRSF18 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    13.7 pg/ml - 10000 pg/ml
  • 复检

    94 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture extracts 93.49 83% - 103%
    Tissue Extracts 94.92 82% - 102%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s TNFRSF18 (GITR) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TNFRSF18 in cell lysates and tissue lysates.

    This assay employs an antibody specific for Human TNFRSF18 coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFRSF18 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNFRSF18 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF18 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    2x Cell lysate buffer 1 x 5ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    5X Sample Diluent Buffer 1 x 10ml
    Biotinylated anti-Human TNFRSF18 2 vials
    Recombinant Human TNFRSF18 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
    TNFRSF18 Microplate (12 x 8 wells) 1 unit
  • 研究领域
  • 相关性
    TNFRSF18 or GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18) originally identified in the mouse by comparing untreated and dexamethasone-treated murine T cell hybridoma cells. Human GITR was subsequently identified by searching an expressed sequence tag database. GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR appears to be highly expressed on CD4+CD25+ T regulatory cells and has been shown to induce NF-kB activation through TRAF2/NIK signaling. GITR has been proposed to be involved in the development of regulatory T cells and to regulate the activity of Th1 subsets. GITR binds GITRL, a TNF superfamily ligand expressed on endothelial cells, dendritic cells, macrophages, and B cells.
  • 别名
    • Activation inducible TNFR family receptor
    • AITR
    • GITR
    • GITR D
    • Glucocorticoid induced TNFR related protein
    • Tumor necrosis factor receptor superfamily member 18
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100528

实验方案

文献

ab100528 has not yet been referenced specifically in any publications.

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