人Albumin ELISA试剂盒(ab179887)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.1 ng/ml
- Range: 0.7 ng/ml - 45 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Cynomolgus monkey
概述
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产品名称
人Albumin ELISA试剂盒
参阅全部 Albumin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 5.2% 批次间 样品 n Mean SD CV% Serum 3 7.6% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.1 ng/ml -
范围
0.7 ng/ml - 45 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 104 104% - 105% Serum 100 92% - 110% Hep Plasma 98 89% - 105% EDTA Plasma 104 97% - 109% Cit plasma 101 100% - 103% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human, Cynomolgus monkey -
产品概述
As of December 12, 2019 Human Albumin ELISA kit has been re-developed with new recombinant monoclonal antibodies to provide improved performance and consistency.
Human Albumin ELISA kit (ab179887) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Albumin protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Albumin with 100 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes native human Albumin protein in serum, plasma, and cell culture supernatant samples only. Urine, saliva, cell and tissue extract samples have not been tested with this kit.
SPECIES REACTIVITY
This kit recognizes human Albumin and Cynomolgus monkey albumin protein. If you require a kit that is human-specific, please see ab227933.
Other species reactivity was determined by measuring 1:4,000,000 serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse, rat, Cow.
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说明
Albumin (ALB), the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. It also serves as a major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 1 x 384 tests 10X Human Albumin Capture Antibody 1 x 600µl 1 x 600µl 10X Human Albumin Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 1 unit Antibody Diluent 4BI 1 x 6ml 1 x 6ml Native Human Serum Albumin protein (Lyophilized) (ab205808) 2 vials 2 vials Plate Seals 1 unit 1 unit Sample Diluent NS (ab193972) 2 x 50ml 1 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 0 x 0 unit Stop Solution 1 x 12ml 2 x 12ml TMB Development Solution 1 x 12ml 2 x 12ml -
研究领域
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功能
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
组织特异性
Plasma. -
疾病相关
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻译后修饰
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
细胞定位
Secreted. - Information by UniProt
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别名
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
数据库链接
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: A2V9Z4 Cynomolgus monkey
- SwissProt: P02768 Human
- Unigene: 418167 Human
- Unigene: 592379 Human
相关产品
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Alternative Versions
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ELISA kits
图片
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Interpolated concentration of native Albumin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are human serum 1:1,000,000. The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Example of human Albumin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Standard Curve comparison between the original human Albumin SimpleStep ELISA kit current human Albumin SimpleStep ELISA kit.
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Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Albumin standard curve in Sample Diluent NS. The Albumin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Albumin were measured in duplicates, interpolated from the Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:4,000,000, plasma (EDTA) 1:4,000,000, plasma (citrate) 1:8,000,000, and plasma (heparin) 1:8,000,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 31.5 mg/mL in serum, 75.4 mg/mL in plasma (EDTA), 35.5 mg/mL in plasma (citrate) and 41.3 mg/mL in plasma (heparin).
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 23.4 mg/mL with a range of 10.5 – 37.1 mg/mL.
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Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Spiked Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab179887 被引用在 15 文献中.
- Farooqi HMU et al. Study of the Anticancer Potential of Plant Extracts Using Liver Tumor Microphysiological System. Life (Basel) 12:N/A (2022). PubMed: 35207423
- Imamura S et al. In vitro culture at 39 °C during hepatic maturation of human ES cells facilitates hepatocyte-like cell functions. Sci Rep 12:5155 (2022). PubMed: 35338220
- Shi Q et al. Co-Culture of Human Primary Hepatocytes and Nonparenchymal Liver Cells in the Emulate® Liver-Chip for the Study of Drug-Induced Liver Injury. Curr Protoc 2:e478 (2022). PubMed: 35790095
- Khati V et al. Indirect 3D Bioprinting of a Robust Trilobular Hepatic Construct with Decellularized Liver Matrix Hydrogel. Bioengineering (Basel) 9:N/A (2022). PubMed: 36354514
- Ewart L et al. Performance assessment and economic analysis of a human Liver-Chip for predictive toxicology. Commun Med (Lond) 2:154 (2022). PubMed: 36473994