人RANTES ELISA试剂盒(ab100633)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人RANTES ELISA试剂盒
参阅全部 RANTES 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 3 pg/ml -
范围
2.74 pg/ml - 2000 pg/ml -
回收率
97 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 102.6 87% - 108% Serum 96.74 85% - 104% Plasma 94.76 84% - 103% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s RANTES Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human RANTES in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human RANTES coated on a 96-well plate. Standards and samples are pipetted into the wells and RANTES present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human RANTES antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANTES bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human RANTES 2 vials RANTES Microplate (12 x 8 wells) 1 unit Recombinant Human RANTES Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. -
组织特异性
T-cell and macrophage specific. -
序列相似性
Belongs to the intercrine beta (chemokine CC) family. -
翻译后修饰
N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity. -
细胞定位
Secreted. - Information by UniProt
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别名
- Beta chemokine RANTES
- Beta chemokine RANTES precursor
- C C motif chemokine 5
see all -
数据库链接
- Entrez Gene: 6352 Human
- Omim: 187011 Human
- SwissProt: P13501 Human
- Unigene: 514821 Human
图片
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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RANTES measured in cell supernatants showing quantity (pg) per mL of tested sample. Samples were diluted 1-8 fold.
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RANTES measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 6-54 fold.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab100633 被引用在 6 文献中.
- Kordulewska N et al. Osthole Regulates Secretion of Pro-Inflammatory Cytokines and Expression of TLR2 and NF-κB in Normal Human Keratinocytes and Fibroblasts. J Inflamm Res 15:1501-1519 (2022). PubMed: 35261546
- Baruah V et al. Prognostic, clinical, and therapeutic importance of RANTES-CCR5 axis in hepatitis A infection: A multiapproach study. J Med Virol 93:3656-3665 (2021). PubMed: 32975838
- Kordulewska NK et al. Osthole Inhibits Expression of Genes Associated with Toll-like Receptor 2 Signaling Pathway in an Organotypic 3D Skin Model of Human Epidermis with Atopic Dermatitis. Cells 11:N/A (2021). PubMed: 35011650
- Sultanova A et al. HHV-6 Infection and Chemokine RANTES Signaling Pathway Disturbance in Patients with Autoimmune Thyroiditis. Viruses 12:N/A (2020). PubMed: 32604892
- Islam M et al. Significance of RANTES-CCR5 axis and linked downstream immunomodulation in Dengue pathogenesis: A study from Guwahati, India. J Med Virol 91:2066-2073 (2019). PubMed: 31368534
- Bujarbaruah D et al. RANTES levels as a determinant of falciparum malaria severity or recovery. Parasite Immunol 39:N/A (2017). PubMed: 28686299