人Procalcitonin ELISA试剂盒(ab100630)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 27.43 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Procalcitonin ELISA试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 20 pg/ml -
范围
27.43 pg/ml - 20000 pg/ml -
回收率
83 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 100.5 80% - 111% Plasma 72.35 65% - 88% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Procalcitonin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Procalcitonin in plasma and cell culture supernatants. (Human Procalcitonin concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human Procalcitonin coated on a 96-well plate. Standards and samples are pipetted into the wells and Procalcitonin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Procalcitonin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Procalcitonin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human Procalcitonin in normal serum with ab100630, only in serum spiked with Human Procalcitonin.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human Procalcitonin 2 vials Procalcitonin Microplate (12 x 8 wells) 1 unit Recombinant Human Procalcitonin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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数据库链接
- Entrez Gene: 796 Human
- SwissProt: P01258 Human
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab100630 被引用在 7 文献中.
- Mohany KM et al. Circulating miR-15b, Annexin A1, procalcitonin and interleukin-6 levels differentiate children with metabolically unhealthy obesity from those with metabolically healthy obesity: A case-control study. Exp Ther Med 23:403 (2022). PubMed: 35637648
- Rosado-Franco JJ et al. Recombinant Fasciola hepatica Fatty Acid Binding Protein as a Novel Anti-Inflammatory Biotherapeutic Drug in an Acute Gram-Negative Nonhuman Primate Sepsis Model. Microbiol Spectr 9:e0191021 (2021). PubMed: 34937173
- Rosado-Franco JJ et al. Outlining key inflammation-associated parameters during early phase of an experimental gram-negative sepsis model in rhesus macaques (Macaca mulatta). Animal Model Exp Med 2:326-333 (2019). PubMed: 31942564
- Ingram JR et al. Levels of wound calprotectin and other inflammatory biomarkers aid in deciding which patients with a diabetic foot ulcer need antibiotic therapy (INDUCE study). Diabet Med 35:255-261 (2018). PubMed: 28734103
- Wan Y et al. Adjuvant rhubarb alleviates organs dysfunction and inhibits inflammation in heat stroke. Exp Ther Med 16:1493-1498 (2018). PubMed: 30116399
- Wan B et al. Rhubarb vs. glycerin enema for treatment of critically ill patients with intra-abdominal hypertension. Exp Ther Med 14:855-861 (2017). PubMed: 28673010
- Nakahira K et al. Circulating mitochondrial DNA in patients in the ICU as a marker of mortality: derivation and validation. PLoS Med 10:e1001577; discussion e1001577 (2013). PubMed: 24391478