人PMN Elastase ELISA试剂盒(ab119553)
Key features and details
- Sensitivity: 1.98 pg/ml
- Range: 0.16 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人PMN Elastase ELISA试剂盒
参阅全部 Neutrophil Elastase 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 4.8% 批次间 样品 n Mean SD CV% Overall 5.6% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
1.98 pg/ml -
范围
0.16 ng/ml - 10 ng/ml -
回收率
104 %
特定样本回收率 样品类型 平均% 范围 Plasma 104 96% - 110% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s PMN Elastase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human PMN Elastase concentrations in Cell culture supernatants, plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma.
PMN Elastase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated PMN Elastase detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the PMN Elastase amount of sample captured in plate.
The capture antibody in the Human PMN-Elastase ELISA kit is specific to PMN elastase, while the detection antibody is specific to alpha1-PI, hence the kit is specific to the PMN Elastase/alpha1-PI complex.
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平台
Microplate
性能
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存放说明
Please refer to protocols. -
组件 1 x 96 tests Adhesive Films 2 units Control high 1 vial Control low 1 vial HRP-Conjugate anti-alpha1-PI polyclonal antibody 1 x 16ml Human PMN Elastase - alpha1-PI complex Standard, lyophilized, (10 ng/mL upon reconstitution) 1 vial Microplate coated with polyclonal antibody to Human PMN Elastase (12 x 8 wells) 1 unit Sample Diluent 1 x 50ml Stop Solution 1 x 7ml TMB Substrate Solution 1 x 22ml Wash Buffer Concentrate (10x) 1 x 50ml -
研究领域
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功能
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. -
组织特异性
Bone marrow cells. -
疾病相关
Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. -
序列相似性
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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别名
- Bone marrow serine protease
- ELA2
- ELANE
see all -
数据库链接
- Entrez Gene: 1991 Human
- Omim: 130130 Human
- SwissProt: P08246 Human
- Unigene: 99863 Human
图片
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab119553 被引用在 28 文献中.
- Louis Sam Titus ASC et al. Resistin, Elastase, and Lactoferrin as Potential Plasma Biomarkers of Pediatric Inflammatory Bowel Disease Based on Comprehensive Proteomic Screens. Mol Cell Proteomics 22:100487 (2023). PubMed: 36549591
- Lodge KM et al. Hypoxia Increases the Potential for Neutrophil-mediated Endothelial Damage in Chronic Obstructive Pulmonary Disease. Am J Respir Crit Care Med 205:903-916 (2022). PubMed: 35044899
- Cedervall J et al. Neutrophil extracellular traps promote cancer-associated inflammation and myocardial stress. Oncoimmunology 11:2049487 (2022). PubMed: 35309730
- D'Amato M et al. Investigating the Link between Alpha-1 Antitrypsin and Human Neutrophil Elastase in Bronchoalveolar Lavage Fluid of COVID-19 Patients. Curr Issues Mol Biol 44:2122-2138 (2022). PubMed: 35678672
- Fungmongkonsatean T et al. Hemocompatibility Evaluation of Thai Bombyx mori Silk Fibroin and Its Improvement with Low Molecular Weight Heparin Immobilization. Polymers (Basel) 14:N/A (2022). PubMed: 35890719