人PLGF ELISA试剂盒(ab100629)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 1.372 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人PLGF ELISA试剂盒
参阅全部 PLGF 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 2 pg/ml -
范围
1.372 pg/ml - 1000 pg/ml -
回收率
89 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 103.4 75% - 115% Serum 88.09 79% - 96% Plasma 75.69 68% - 93% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s PLGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PLGF in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human PLGF coated on a 96-well plate. Standards and samples are pipetted into the wells and PLGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PLGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated anti-Human PLGF 2 vials PLGF Microplate (12 x 8 wells) 1 unit Recombinant Human PLGF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. -
组织特异性
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas. -
序列相似性
Belongs to the PDGF/VEGF growth factor family. -
结构域
Isoform PlGF-2 contains a basic insert which acts as a cell retention signal. -
翻译后修饰
N-glycosylated. -
细胞定位
Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. - Information by UniProt
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别名
- D12S1900
- Pgf
- PGFL
see all -
数据库链接
- Entrez Gene: 5228 Human
- Omim: 601121 Human
- SwissProt: P49763 Human
- Unigene: 252820 Human
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab100629 被引用在 7 文献中.
- Xie Y et al. Quantitative proteomics reveal three potential biomarkers for risk assessment of acute myocardial infarction. Bioengineered 13:4939-4950 (2022). PubMed: 35156527
- Buell-Acosta JD et al. Maternal Fibroblast Growth Factor 21 Levels Decrease during Early Pregnancy in Normotensive Pregnant Women but Are Higher in Preeclamptic Women-A Longitudinal Study. Cells 11:N/A (2022). PubMed: 35883694
- Cubro H et al. Mechanisms of vascular dysfunction in the interleukin-10-deficient murine model of preeclampsia indicate nitric oxide dysregulation. Kidney Int 99:646-656 (2021). PubMed: 33144212
- Rao SL et al. Altered level of salivary placental growth factor is associated with preeclampsia. Placenta 90:118-120 (2020). PubMed: 32056542
- Tan L et al. Sublethal heat treatment of hepatocellular carcinoma promotes intrahepatic metastasis and stemness in a VEGFR1-dependent manner. Cancer Lett 460:29-40 (2019). PubMed: 31173855
- Cheung WY et al. Efficient Nonviral Transfection of Human Bone Marrow Mesenchymal Stromal Cells Shown Using Placental Growth Factor Overexpression. Stem Cells Int 2018:1310904 (2018). PubMed: 30675166
- Garcés MF et al. Maternal Serum Meteorin Levels and the Risk of Preeclampsia. PLoS One 10:e0131013 (2015). PubMed: 26121675