人MICA ELISA试剂盒(ab59569)
Key features and details
- Sensitivity: 123 pg/ml
- Range: 156.25 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人MICA ELISA试剂盒
参阅全部 MICA 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% A 6 2214.6pg/ml 50.3 2.3% B 6 1102.1pg/ml 39.5 3.6% C 6 641.6pg/ml 47.7 7.4% 批次间 样品 n Mean SD CV% A 18 2416pg/ml 187.1 7.7% B 16 1200pg/ml 105.2 8.8% C 18 627pg/ml 50.9 8.1% -
样品类型
Cell culture supernatant -
检测类型
Sandwich (quantitative) -
灵敏度
= 123 pg/ml -
范围
156.25 pg/ml - 5000 pg/ml -
检测时间
3h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s MICA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of MHC class 1 chain-related gene A glycoprotein, in cell culture supernatants and buffered solutions
A monoclonal antibody specific for MICA has been coated onto the wells of the microtiter strips provided. Samples, including standards of known MICA concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for MICA are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of MICA present in the samples.
This kit will recognize both endogenous and recombinant Human MICA.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 2 x 96 tests 1 x 96 tests 1 x 96 tests 2 x 96 tests 1X Standard Diluent Buffer 2 x 25ml 1 x 25ml 1 x 25ml 2 x 25ml 200X Wash Buffer White 2 x 10ml 1 x 10ml 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml 1 x 7ml 1 x 13ml Biotinylated anti-MICA (MHC Class I alpha) Red 2 x 400µl 1 x 400µl 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 23ml 1 x 12ml 1 x 12ml 1 x 23ml MICA Microplate (12 x 8 well strips) 2 units 1 unit 1 unit 2 units MICA Standard (lyophilized) Yellow 4 vials 2 vials 2 vials 4 vials Stop Reagent Black 2 x 11ml 1 x 11ml 1 x 11ml 2 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl 2 x 5µl 4 x 5µl -
研究领域
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相关性
The MHC class I chain-related (MIC) proteins are related to the Major histocompatibility complex (MHC) class I proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. The MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. MICA is present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system. -
细胞定位
Plasma membrane -
别名
- FLJ60820
- HLA class I antigen
- MGC111087
see all -
数据库链接
- Entrez Gene: 4276 Human
- Omim: 600169 Human
- SwissProt: Q29983 Human
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab59569 被引用在 3 文献中.
- Ferrari de Andrade L et al. Inhibition of MICA and MICB Shedding Elicits NK-Cell-Mediated Immunity against Tumors Resistant to Cytotoxic T Cells. Cancer Immunol Res 8:769-780 (2020). PubMed: 32209637
- Formenti SC et al. Radiotherapy induces responses of lung cancer to CTLA-4 blockade. Nat Med 24:1845-1851 (2018). PubMed: 30397353
- Fu D et al. Metabolic perturbation sensitizes human breast cancer to NK cell-mediated cytotoxicity by increasing the expression of MHC class I chain-related A/B. Oncoimmunology 4:e991228 (2015). PubMed: 25949910