Plasma samples from pigs 3-4 months old (25-30 kg body weight) were obtained after overnight fasting.
Assay procedure was performed according to manufacturer instructions. However, standard curve (duplicates) was modified due to the low concentrations observed in fasted pigs. Standard concentrations were 100, 50, 25, 12.5, 6.25, 3.125 and 1.5625 microIU/mL, prepared by a 1:3 dilution of the first standard provided with the product, and consecutive 1:2 dilutions thereafter.
Plasma samples were added to each well by duplicate (100 microL) as suggested by the manufacturer instructions, with no dilution. All remaining steps were performed in accordance with manufacturer instrucitions, with no modificatons. Readings were performed at 450 nm.
Results were as follows:
Control animals: 6.63, 3.65, 1.10 and 4.67 microIU/mL (mean: 4.01±1.15 microIU/mL)
Treatment A: 54.95, 9.29, 8.77, 5.10 microIU/mL (mean: 19.53±11.84).
Assay procedure was performed according to manufacturer instructions. However, standard curve (duplicates) was modified due to the low concentrations observed in fasted pigs. Standard concentrations were 100, 50, 25, 12.5, 6.25, 3.125 and 1.5625 microIU/mL, prepared by a 1:3 dilution of the first standard provided with the product, and consecutive 1:2 dilutions thereafter.
Plasma samples were added to each well by duplicate (100 microL) as suggested by the manufacturer instructions, with no dilution. All remaining steps were performed in accordance with manufacturer instrucitions, with no modificatons. Readings were performed at 450 nm.
Results were as follows:
Control animals: 6.63, 3.65, 1.10 and 4.67 microIU/mL (mean: 4.01±1.15 microIU/mL)
Treatment A: 54.95, 9.29, 8.77, 5.10 microIU/mL (mean: 19.53±11.84).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antonio Rodriguez Sinovas
Verified customer
提交于 Oct 28 2014