人IL13酶联免疫吸附(ELISA)套装(without plates) (ab47353)

概述

  • 产品名称
    人IL13酶联免疫吸附(ELISA)套装(without plates)
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 1.5 pg/ml
  • 范围
    3.21 pg/ml - 100 pg/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-13 Human in vitro ELISA Set is designed for the quantitative measurement of IL-13 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    This ELISA Set is intended to measure human IL-13 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    The IL-13 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human IL13.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Reagents

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 5 x 96 tests 10 x 96 tests 15 x 96 tests
    Detection biotinylated anti-IL-13 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
    IL-13 Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml
    IL13 standard Yellow 5 vials 10 vials 15 vials
    Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials
    Streptavidin-HRP 1 vial 2 vials 3 vials
  • 研究领域
  • 相关性
    Human IL13 was originally identified by differential screening of an anti CD28 activated human peripheral blood mononuclear cell cDNA library as an induction specific novel cytokine. It was also isolated from cDNA libraries of human T cell clones using the murine IL13 (P600) cDNA as a probe. Human IL13, a pleiotropic cytokine, is produced by activated Th0, Th1 like, Th2 like, and CD8 T cells. The gene for human IL13 maps to chromosome 5 and is closely linked to the genes for IL3, IL4, IL5, and GMCSF. IL13 inhibits proinflammatory cytokine production and stimulates antibody production. It induces proliferation in the human pre myeloid cell line TF1. IL13 has multiple effects on the differentiation and functions of monocytes and macrophages. It suppresses cytotoxic functions and induces changes in the morphology of human monocytes and in the phenotype of human monocytes and B cells by upregulating MHC class II expression. IL13 will also decrease the production of nitric oxide by activated murine macrophages, leading to impaired parasiticidal activity. Human and mouse interleukin 13 share approximately 58% amino acid sequence identity. Although human and mouse IL13 are equally active on human cells, human IL13 is much less active than mouse IL13 on mouse cells. Human IL13 and human IL4 also share approximately 30% sequence homology and have similar biological functions.
  • 细胞定位
    Secreted
  • 别名
    • ALRH
    • BHR1
    • Interleukin 13
    • P600
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab47353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • IL-13 Standard curve ranging from 3.12 to 100 pg/mL

文献

ab47353 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your call last week and for your patience while I have been in touch with the lab about ab47353. Both the capture and detection antibodies are produced in mouse. I hope this information will be useful, but please let me know if you have a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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