人IL-2 Receptor ELISA试剂盒(ab46036)
Key features and details
- Sensitivity: 32.5 pg/ml
- Range: 68.75 pg/ml - 2200 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-2 Receptor ELISA试剂盒
参阅全部 IL-2 Receptor alpha 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 4.5% 批次间 样品 n Mean SD CV% 1 6 1594pg/ml 184 11.6% 2 6 1528pg/ml 135 8.8% 3 6 1705pg/ml 204 12% 4 6 1124pg/ml 171 15.2% 5 6 2305pg/ml 165 7.2% 6 6 1824pg/ml 126 69% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 32.5 pg/ml -
范围
68.75 pg/ml - 2200 pg/ml -
回收率
87 %
特定样本回收率 样品类型 平均% 范围 Serum 99 86% - 122% -
检测时间
3h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-2 Receptor Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 Receptor in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant Human IL-2 Receptor.
A monoclonal antibody specific for IL-2 Receptor has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 Receptor concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 Receptor are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 Receptor present in the samples.
This kit will recognize both endogenous and recombinant Human IL-2 Receptor.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 2 x 96 tests 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 1 x 25ml 200X Wash Buffer White 2 x 10ml 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml 1 x 13ml Biotinylated anti-IL-2 Receptor Red 2 x 0.4ml 1 x 0.4ml 2 x 0.4ml Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 23ml 1 x 12ml 1 x 23ml IL-2 Receptor Control Silver 4 vials 2 vials 4 vials IL-2 Receptor Microplate (12 x 8 well strips) 2 units 1 unit 2 units Plastic Plate Covers 4 units 2 units 4 units Stop Reagent Black 2 x 11ml 1 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl 4 x 5µl -
研究领域
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功能
Receptor for interleukin-2. -
疾病相关
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
序列相似性
Contains 2 Sushi (CCP/SCR) domains. -
细胞定位
Membrane. - Information by UniProt
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别名
- Interleukin 2 receptor alpha chain
- CD25
- CD25 antigen
see all -
数据库链接
- Entrez Gene: 3559 Human
- Omim: 147730 Human
- SwissProt: P01589 Human
- Unigene: 231367 Human
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab46036 被引用在 2 文献中.
- Meng D et al. Effects of VEGFR1+ hematopoietic progenitor cells on pre-metastatic niche formation and in vivo metastasis of breast cancer cells. J Cancer Res Clin Oncol 145:411-427 (2019). PubMed: 30483898
- Ngcobo M & Gqaleni N Evaluation of the immunomodulatory effects of a South African commercial traditional immune booster in human peripheral blood mononuclear cells. BMC Complement Altern Med 16:300 (2016). PubMed: 27550057