人HB EGF ELISA试剂盒(ab100531)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 16.38 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人HB EGF ELISA试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 20 pg/ml -
范围
16.38 pg/ml - 4000 pg/ml -
回收率
90 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 81.22 73% - 90% Plasma 93.73 84% - 103% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s HB EGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HB EGF in plasma and cell culture supernatants. (Human HB EGF concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human HB EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HB EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HB EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HB EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human HB-EGF in normal serum with ab100531, only in serum spiked with Human HB-EGF.
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说明
Optimization may be required with urine samples.
The standard is affinity purified.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human HB EGF 2 vials HB EGF Microplate (12 x 8 wells) 1 unit Recombinant Human HB EGF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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数据库链接
- SwissProt: Q9W7C5 Human
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab100531 被引用在 2 文献中.
- Wang X et al. Sphingosine 1-Phosphate Activation of EGFR As a Novel Target for Meningitic Escherichia coli Penetration of the Blood-Brain Barrier. PLoS Pathog 12:e1005926 (2016). PubMed: 27711202
- Hutchinson KE et al. ERBB activation modulates sensitivity to MEK1/2 inhibition in a subset of driver-negative melanoma. Oncotarget 6:22348-60 (2015). PubMed: 26084293