人Follistatin ELISA试剂盒(ab192147)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 28 pg/ml
- Range: 0.08 ng/ml - 5 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Follistatin ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 8 6.3% 批次间 样品 n Mean SD CV% Overall 3 2.5% -
样品类型
Cell culture supernatant, Serum, EDTA Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
28 pg/ml -
范围
0.08 ng/ml - 5 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 110 99% - 125% Cell culture media 86 85% - 86% EDTA Plasma 92 89% - 95% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Follistatin ELISA Kit (ab192147) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Follistatin protein in cell culture supernatant, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Follistatin with 28 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Follistatin is a single-chain polypeptide consisting of about 344 amino acids. The follistatin protein is encoded by a relatively small gene size of 6 kb and 6 exons with alternative splice variants and post translational modifications to give a variety of molecular weights between 31 – 39 kDa. Follistatin forms a high affinity complex with activin sequestering it from its receptor thus having an inhibitory effect. Follistatin acts to promote muscle growth in mammals by acting as an antagonist to the protein myostatin. Myostatin acts as an inhibitor to muscle growth.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human Follistatin Capture Antibody 1 x 600µl 10X Human Follistatin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Follistatin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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细胞定位
Secreted -
别名
- Activin binding protein
- Follistatin isoform FST317
- FS
- FST
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数据库链接
- Entrez Gene: 10468 Human
- SwissProt: P19883 Human
图片
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Background-subtracted data values (mean +/- SD) are graphed.
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(mean +/- SD, n =2).
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Interpolated values of Follistatin are plotted on the bar graph. No signal was detected in Donors 6, 7 or 8.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab192147 被引用在 1 文献中.
- He BL et al. Follistatin is a novel therapeutic target and biomarker in FLT3/ITD acute myeloid leukemia. EMBO Mol Med 12:e10895 (2020). PubMed: 32134197