人Factor VII ELISA试剂盒(ab108829)
Key features and details
- Sensitivity: 1.8 ng/ml
- Range: 10 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Factor VII ELISA试剂盒
参阅全部 Factor VII 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 4.9% 批次间 样品 n Mean SD CV% Overall 8.6% -
样品类型
Cell culture supernatant, Saliva, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 1.8 ng/ml -
范围
10 ng/ml - 40 ng/ml -
回收率
96 %
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检测时间
4h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Factor VII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor VII concentrations in Human cell culture supernatants, serum and plasma.
A Factor VII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor VII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor VII captured in plate. This assay recognizes FVII and FVIIa.
Get higher sensitivity in only 90 minutes with Human Factor VII ELISA Kit (ab190810) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Factor VII Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Factor VII Microplate (12 x 8 well strips) 1 unit Factor VII Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. -
组织特异性
Plasma. -
疾病相关
Defects in F7 are the cause of factor VII deficiency (FA7D) [MIM:227500]. FA7D is a rare hereditary hemorrhagic disease. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Numerous subjects are completely asymptomatic despite a very low F7 level. -
序列相似性
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
翻译后修饰
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
细胞定位
Secreted. - Information by UniProt
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别名
- Coagulation factor VII
- coagulation factor VII (serum prothrombin conversion accelerator)
- Eptacog alfa
see all -
数据库链接
- Entrez Gene: 2155 Human
- Omim: 613878 Human
- SwissProt: P08709 Human
- Unigene: 36989 Human
相关产品
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SimpleStep ELISA kits
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab108829 被引用在 5 文献中.
- Hu YN et al. A novel compound heterozygous variant linked to hematuria in a family with hereditary factor VII deficiency. J Gene Med 24:e3398 (2022). PubMed: 34786791
- Feser CJ et al. Engineering CRISPR/Cas9 for Multiplexed Recombinant Coagulation Factor Production. Int J Mol Sci 23:N/A (2022). PubMed: 35563479
- Liang Y et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). PubMed: 29599912
- Mashayekhi A et al. Functional and Molecular Characterization of C91S Mutation in the Second Epidermal Growth Factor-Like Domain of Factor VII. Iran J Biotechnol 16:e1813 (2018). PubMed: 30555841
- Mashayekhi A et al. In vitro expression of mutant factor VII proteins and characterization of their clinical significance. Mol Med Rep 17:2738-2742 (2018). PubMed: 29207137