人ENT1多肽(ab48606)
Key features and details
- Purity: > 90% HPLC
- Suitable for: Blocking
描述
-
产品名称
人ENT1多肽 -
纯度
> 90 % HPLC. -
Accession
-
无动物成分
No -
性质
Synthetic -
-
种属
Human
-
技术指标
Our Abpromise guarantee covers the use of ab48606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Blocking
-
形式
Liquid -
补充说明
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
常规信息
-
别名
- Equilibrative NBMPR-sensitive nucleoside transporter
- equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter
- Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
see all -
功能
Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs). -
组织特异性
Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen. -
序列相似性
Belongs to the SLC29A transporter family. -
翻译后修饰
Glycosylated. -
细胞定位
Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells. - Information by UniProt
图片
-
All lanes : Anti-ENT1 antibody (ab48607) at 2 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : Human heart tissue lysate - total protein (ab29431) withHuman ENT1 peptide (ab48606) at 2 µg/ml
Lane 4 : Human brain tissue lysate - total protein (ab29466) withHuman ENT1 peptide (ab48606) at 2 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Exposure time: 4 minutes
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (0)
ab48606 尚未被引用在任何文献中。