人Annexin V ELISA试剂盒(ab119503)

概述

  • 产品名称
    人Annexin V ELISA试剂盒
    参阅全部 Annexin V 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall 11%
  • 样品类型
    Cell culture supernatant
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    0.33 ng/ml
  • 范围
    0.78 ng/ml - 50 ng/ml
  • 复检

    85 %

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Annexin V Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Annexin V concentrations in cell culture supernatant and serum.

    Annexin V specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated Annexin V detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Annexin V sample captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab119503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119503.

实验方案

文献

ab119503 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you very much for your inquiry.

Once the standard is reconstituted, we recommend using them immediately and then discarding the remaining portion.
We have not performed stability studies regarding the long -term storage of reconsti...

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Once the standard is reconstituted, we recommend using them immediately and then discarding the remaining portion.


We have not performed stability studies regarding the long -term storage of reconstituted standard protein so unfortunate...

Read More

Thank you for contacting us. The reconstitution instructions for the standard are lot specific and will be written on the vial. If for any reason it is not, please let us know your lot number and we will be happy to find that information for you.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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