Application
Western blot
Sample
Mouse Cell lysate - whole cell (NSC34)
Loading amount
10 µg
Specification
NSC34
Gel Running Conditions
Reduced Denaturing (4-12% nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/3000
Detection
Detection method
Licor
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 36 kDa Non-specific: 52 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 24 2010