Anti-HuR / ELAVL1抗体(ab28660)
Key features and details
- Rabbit polyclonal to HuR / ELAVL1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-HuR / ELAVL1抗体
参阅全部 HuR / ELAVL1 一抗 -
描述
兔多克隆抗体to HuR / ELAVL1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chicken, Cow -
免疫原
Synthetic peptide corresponding to Human HuR/ ELAVL1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab28843) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28660于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Another band of approximately 52 kDa is also detected, we are unsure as the identity of this band.
|
说明 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Another band of approximately 52 kDa is also detected, we are unsure as the identity of this band. |
靶标
-
功能
Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the RRM elav family.
Contains 3 RRM (RNA recognition motif) domains. -
翻译后修饰
Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 1994 Human
- Entrez Gene: 15568 Mouse
- Omim: 603466 Human
- SwissProt: Q15717 Human
- SwissProt: P70372 Mouse
- Unigene: 184492 Human
- Unigene: 713744 Human
- Unigene: 119162 Mouse
-
别名
- ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
- ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
- ELAV like 1 antibody
see all
图片
-
All lanes : Anti-HuR / ELAVL1 antibody (ab28660) at 1 µg/ml
Lane 1 : Wild-type SW480 cell lysate
Lane 2 : ELAVL1 knockout SW480 cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : HCT 116 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDaFalse colour image of Western blot: Anti-HuR / ELAVL1 antibody staining at 1 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab28660 was shown to bind specifically to HuR / ELAVL1. A band was observed at 36 kDa in wild-type SW480 cell lysates with no signal observed at this size in ELAVL1 knockout cell line ab270485 (knockout cell lysate ab270508). To generate this image, wild-type and ELAVL1 knockout SW480 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
-
All lanes : Anti-HuR / ELAVL1 antibody (ab28660) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab28660 被引用在 5 文献中.
- Youn YH et al. Primary cilia control translation and the cell cycle in medulloblastoma. Genes Dev 36:737-751 (2022). PubMed: 35798383
- Liu Z et al. Circular RNA cIARS regulates ferroptosis in HCC cells through interacting with RNA binding protein ALKBH5. Cell Death Discov 6:72 (2020). PubMed: 32802409
- Green LC et al. Human antigen R as a therapeutic target in pathological cardiac hypertrophy. JCI Insight 4:N/A (2019). PubMed: 30668549
- Wang F et al. The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis. Cell Res 27:416-439 (2017). PubMed: 28244490
- Workman E et al. U1A regulates 3' processing of the survival motor neuron mRNA. J Biol Chem 289:3703-12 (2014). PubMed: 24362020