Anti-Hsp90 beta (phospho S226)抗体(ab63562)
Key features and details
- Rabbit polyclonal to Hsp90 beta (phospho S226)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Hsp90 beta (phospho S226)抗体
参阅全部 Hsp90 beta 一抗 -
描述
兔多克隆抗体to Hsp90 beta (phospho S226) -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide corresponding to Human Hsp90 beta (phospho S226).
Database link: P08238 -
阳性对照
- NIH/3T3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63562于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
|
ICC/IF |
1/500 - 1/1000.
|
说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
ICC/IF
1/500 - 1/1000. |
靶标
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功能
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
序列相似性
Belongs to the heat shock protein 90 family. -
结构域
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins. -
翻译后修饰
Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 3326 Human
- Entrez Gene: 15516 Mouse
- Entrez Gene: 301252 Rat
- Omim: 140572 Human
- SwissProt: P08238 Human
- SwissProt: P11499 Mouse
- SwissProt: P34058 Rat
- Unigene: 509736 Human
see all -
别名
- 90 kda heat shock protein beta HSP90 beta antibody
- D6S182 antibody
- FLJ26984 antibody
see all
图片
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All lanes : Anti-Hsp90 beta (phospho S226) antibody (ab63562) at 1/500 dilution
Lane 1 : extracts from A549 cells, treated
with serum (20%, 15mins)
Lane 2 : extracts from A549 cells, treated
with serum (20%, 15mins) with immunizing phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa -
ab63562 at 1/500 dilution staining NIH/3T3 cells, without (left) and with (right) immunizing peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab63562 被引用在 1 文献中.
- Wang H et al. Hsp90a forms a stable complex at the cilium neck for the interaction of signalling molecules in IGF-1 receptor signalling. J Cell Sci 128:100-8 (2015). PubMed: 25359884