Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk (TBS-tween)
Dilution
1/1000
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
4 minute(s) and 10 second(s)
Bands
Specific: 70 kDa
Additional data
Additional Notes
Homogenates were prepared in a buffer containing 50 mM Tris
(pH 7.4), 150 mM NaCl, and protease inhibitors.
Note that the uploaded image was used in following publication "pubmed ID 25181601"
Abcam response
The customer informed us, that the Hsp70 antibody used in the J Proteome Res Paper is ab31010. The customer has confirmed that there is a typo error in the paper and that the Hsp70 antibody should have been included along with the other antibodies from Abcam mentioned in the Materials&Methods section.
PubMed ID
25181601: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 09 2016