Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Skin fibroblasts)
Specification
Skin fibroblasts
Fixative
Formaldehyde
Permeabilization
Yes - saponin
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C
Secondary antibody
Name
Non-Abcam antibody was used: AF488 goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
The labelling is poor and appears in the nucleus. We have make an other ICF : Fixation in MetOH and permeabilisation in Triton X-100 0.3% with a blocking step in BSA 2% and the labelling done the same result that PFA.
Abcam response
The customer tested this antibody in the previously untested application of immunohistochemistry. Unfortunately it seems that this antibody is unsuitable for immunocytochemistry and we can therefore not recommend this antibody for this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Apr 02 2012