Anti-Hsp40抗体(ab69402)
Key features and details
- Rabbit polyclonal to Hsp40
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-Hsp40抗体
参阅全部 Hsp40 一抗 -
描述
兔多克隆抗体to Hsp40 -
宿主
Rabbit -
特异性
This antibody detects a ~40 kDa protein, corresponding to the apparent molecular mass of Hsp40 on SDS-PAGE immunoblots. It does not detect DnaJ in E.coli lysates.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Recombinant fragment
不与反应: Escherichia coli -
免疫原
Recombinant human Hsp40
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阳性对照
- WB: Heat shocked HeLa cells and 3T3 cells, PC-12 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab69402于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
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说明 |
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WB
1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa). |
靶标
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功能
Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. -
序列相似性
Contains 1 J domain. -
细胞定位
Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. - Information by UniProt
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数据库链接
- Entrez Gene: 3337 Human
- Entrez Gene: 81489 Mouse
- Entrez Gene: 361384 Rat
- Omim: 604572 Human
- SwissProt: P25685 Human
- SwissProt: Q9QYJ3 Mouse
- Unigene: 515210 Human
- Unigene: 282092 Mouse
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别名
- DnaJ (Hsp40) homolog subfmaily B member 1 antibody
- DNAJ 1 antibody
- DNAJ B1 antibody
see all
图片
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All lanes : Anti-Hsp40 antibody (ab69402) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Hsp40 recombinant protein
Lane 3 : DnaJ recombinant protein
Lane 4 : Cell lysates prepared from heat shocked hela cells
Lane 5 : Cell lysates prepared from heat shocked 3T3 cells
Lane 6 : Cell lysates prepared from PC-12 cells
Predicted band size: 38 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab69402 被引用在 13 文献中.
- Gulati R et al. Phosphorylation-Mediated Activation of β-Catenin-TCF4-CEGRs/ALCDs Pathway Is an Essential Event in Development of Aggressive Hepatoblastoma. Cancers (Basel) 14:N/A (2022). PubMed: 36551548
- Capitano ML et al. Mitigating oxygen stress enhances aged mouse hematopoietic stem cell numbers and function. J Clin Invest 131:N/A (2021). PubMed: 33393491
- Zhang Y et al. Proteomic Profiling of Purified Rabies Virus Particles. Virol Sin 35:143-155 (2020). PubMed: 31429011
- Nadeem A et al. Beta-sheet-specific interactions with heat shock proteins define a mechanism of delayed tumor cell death in response to HAMLET. J Mol Biol 431:2612-2627 (2019). PubMed: 31082436
- Zhang W et al. Cellular DNAJA3, a Novel VP1-Interacting Protein, Inhibits Foot-and-Mouth Disease Virus Replication by Inducing Lysosomal Degradation of VP1 and Attenuating Its Antagonistic Role in the Beta Interferon Signaling Pathway. J Virol 93:N/A (2019). PubMed: 30996089