Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293)
Gel Running Conditions
Reduced Denaturing (10% Acrylamide)
Loading amount
20 µg
Specification
HEK-293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS-Tween 20 0.1%
Dilution
1/2000
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: ECL Anti-Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL prime
Exposure
1 second(s)
Bands
Specific: 105 kDa
Positive control
20 nanograms of his-tagged HSPH1/Hsp105 purified from E. coli were added in a second lane to serve as positive control
Additional data
Additional Notes
Excellent monoclonal antibody, no non-specific band to speak of. It recognized a band of the appropriate molecular weight (105 kDa) in HEK-293 whole cell extract as well as the recombinant protein purified from bacteria, so you know it is specific ! I have no problem believing this antibody would work in practically all applications.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 20 2013