Application
Western blot
Sample
Human Cell lysate - whole cell (primary cells)
Loading amount
20 µg
Specification
primary cells
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: NA9340V ECL anti-rabbit HRP in TBST + 5%milk
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
24 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: N/A kDa Non-specific: various kDa
Additional data
Abcam response
Thank you for your feedback. We are very sorry that this antibody did not work with the provided protocol. Due to the nature of antibodies protocol optimization sometime are necessary for an antibody to work in experiments. We have successfully used this antibody in WB and we are sure that with some changes in protocol e.g. increasing primary dilutions and by adding protease inhibitors in buffers success can be achieved.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 11 2011