Application
Western blot
Sample
Xenopus laevis Tissue lysate - other (stage 18 lane 1,2: cytosol lane 3,4: chromatin fra)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
0 cells
Specification
stage 18 lane 1,2: cytosol lane 3,4: chromatin fra
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1xTBS 0.05%Tween 2%BSA
Concentration
0.5 µg/ml
Detection
Detection method
Li COR
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
Probably, the antibodies can be used more diluted. We dilute the antibodies in 1xTBS0.05% tween, 2%BSA and reuse them several times. In-between use, we store the solution at -20°C.
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提交于 Aug 07 2015