Application
Western blot
Sample
Human Cell lysate - whole cell (multiple myeloma cell)
Loading amount
50 µg
Specification
multiple myeloma cell
Gel Running Conditions
Reduced Denaturing (10-20% tris glycine gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Secondary antibody
Name
Non-Abcam antibody was used: biorad secondary
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 17 kDa
Additional data
Additional Notes
KNS11/TKO cells are multiple myeloma derived cell lines (Lauring et al: Blood 2008, 111, 856-854).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 21 2012