Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Cell (ovaries)
Permeabilization
Yes - PBST
Specification
ovaries
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Jackson ImmunoResearch
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
This is a normal staining. In the earlier stages of oogenesis when the karyosome has just formed there is DNA with spots of marks. Although it is diffuse it is comparable to that of other acetylation stainings (like H2BK20ac and H3K9ac) which were done using Abcam antibodies.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 24 2015