Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Rat Cell lysate - whole cell (Primary Neurons)
Specification
Primary Neurons
Treatment
50 nM TSA for 24h
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Bands
Specific: ~12 kDa
Exposure
30 second(s)
Positive control
Lane 1 - Control
Lane 2 - 50 nM TSA for 24h
Additional data
Additional Notes
Lane 1 - Control
Lane 2 - 50 nM TSA for 24h
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 29 2013