Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3MEF)
Gel Running Conditions
Reduced Denaturing (12.5% acrylamide-Bis gel)
Loading amount
50000 cells
Specification
3T3MEF
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5%milk in PBST
Dilution
1/6000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG [HRP] (GE Healthcare, LNA934V
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 17 kDa
Positive control
H3
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 14 2015