Application
Western blot
Sample
Pig Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (17,5)
Loading amount
20 µg
Specification
Liver
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL prime
Exposure
3 minute(s) and 15 second(s)
Bands
Specific: 15 kDa Non-specific: 25, 30, 40 kDa
Positive control
anti Histone H3 antibody
Additional data
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with pig and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 28 2017