Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10% PAGE)
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
13 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1x TBS
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Chemiluminescence
Bands
Specific: 15 kDa Non-specific: 37, 65 kDa
Exposure
30 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 30 2015