Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (S288c background)
Loading amount
40 µg
Specification
S288c background
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/250
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Host species: Goat
Clonality: Polyclonal
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 18 kDa
Positive control
Antibody Ab9050 specific to H3K36me3
Negative control
Yeast with point mutation K36A
Additional data
Additional Notes
The antibody is not specific to H3K36 methylation.
Abcam response
This negative Abreview is currently being investigated by Abcam Technical Support. Please check here for an update response from Abcam.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 08 2007