Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (WT and set2 delete S288c S. cerevisiae)
Loading amount
5 µg
Specification
WT and set2 delete S288c S. cerevisiae
Gel Running Conditions
Reduced Denaturing (12.5% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% MILK in TBST
Secondary antibody
Name
Dilution
1/15000
Detection
Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: ~15 kDa
Positive control
Antibody Ab9050 specific to H3K36me3
Negative control
set2 deletion mutant
Additional data
Additional Notes
antibody does not exhibit specificity on western blots. the band does not go away in a set2 deletion mutant.
Abcam response
Thank you for your valuable feedback. It seems that this antibody does not specifically recognize Histone H3 K36 Mono Methyl in WB in S. cerevisiae under these conditions. Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Marc Meneghini
Verified customer
提交于 Oct 11 2010