Application
Western blot
Sample
Caenorhabditis elegans Tissue lysate - nuclear (C. elegans whole body)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
4.5 µg
Specification
C. elegans whole body
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 0.1% Tween 20 + 5% BSA
Concentration
5 µg/ml
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-mouse IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Recombinant histone H3 was used as negative control as labeled.
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 15 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 29 2013