Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Sample
Mouse Cell lysate - whole cell (MEL)
Specification
MEL
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% skim milk
Dilution
1/2500
Secondary antibody
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (HRP)
Detection
Detection method
ECL
Bands
Specific: 17 kDa Non-specific: 14 kDa
Exposure
1 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 08 2015