Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (IMR32-Human Neuroblastoma)
Specification
IMR32-Human Neuroblastoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.2% BSA containing TBST
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Goat-Anti Rabbit IgG-HRP, sigma
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
Perkin Elmer ECL reagent
Bands
Specific: 17 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
nil
Positive control
nil
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 26 2013