Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10 %)
Sample
Rat Cell lysate - nuclear (Brain-striatum and cortex)
Specification
Brain-striatum and cortex
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5 % BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: ZDR-5307
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/5000
Detection
Detection method
ECL+
Bands
Specific: 15+ kDa Non-specific: 15- kDa
Exposure
30 second(s)
Negative control
NA
Positive control
NA
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Tianyu Zhang
Verified customer
提交于 Jan 21 2015